Question
Which of the following is the correct sequence of
reagents given below used in the Gram staining process? (A) lodine (B) Crystal violet (C) Safranın (D) Alcohol Choose the correct answer from the options given below:Solution
The correct order of reagents in Gram staining is: (B) Crystal violet: Primary stain. (A) Iodine: Mordant, which fixes the dye to the cell wall. (D) Alcohol: Decolorizer that removes the primary dye from Gram-negative cells. (C) Safranin: Counterstain that stains Gram-negative cells pink.
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