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    Question

    Which of the following is the correct sequence of

    reagents given below used in the Gram staining process? (A) lodine (B) Crystal violet (C) Safran─▒n (D) Alcohol Choose the correct answer from the options given below:
    A (A), (B) (C) (D) Correct Answer Incorrect Answer
    B (A), (C), (B), (D) Correct Answer Incorrect Answer
    C (B), (A), (D), (C) Correct Answer Incorrect Answer
    D (C), (B), (D). (A) Correct Answer Incorrect Answer

    Solution

    The correct order of reagents in Gram staining is: (B) Crystal violet: Primary stain. (A) Iodine: Mordant, which fixes the dye to the cell wall. (D) Alcohol: Decolorizer that removes the primary dye from Gram-negative cells. (C) Safranin: Counterstain that stains Gram-negative cells pink.

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