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    Question

    Which enzyme is responsible for removing RNA primers

    during DNA replication?
    A Helicase Correct Answer Incorrect Answer
    B DNA polymerase I Correct Answer Incorrect Answer
    C DNA polymerase III Correct Answer Incorrect Answer
    D Ligase Correct Answer Incorrect Answer

    Solution

    During DNA replication, RNA primers are required to initiate the synthesis of new DNA strands. These primers are later removed and replaced with DNA. DNA polymerase I in prokaryotes (like E. coli) is responsible for removing RNA primers and replacing them with DNA using its 5' → 3' exonuclease activity. This enzyme also has polymerase activity to fill the gaps left after primer removal.

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