Question
The efficiency (adequacy) of pasteurization of milk is
tested bySolution
1. Science Behind the Test: a. Alkaline phosphatase enzyme naturally present in raw milk b. Destroyed at 72°C for 15 sec (HTST standards) c. Residual enzyme indicates inadequate pasteurization 2. Testing Protocol: a. Add disodium phenyl phosphate + buffer b. Incubate at 37°C ± 1°C for 1 hour c. Measure liberated phenol (colorimetric) 3. Regulatory Standards: a. FSSAI: ≤ 350 mU/L for pasteurized milk b. Codex Alimentarius: Complete inactivation required 4. Comparative Analysis: 
5. Industry Practice: a. Mandatory for all dairy processors b. Automated test systems (e.g., MilkoScan FT3) widely adopted Technical Note: False positives may occur if milk is contaminated with microbial phosphatases. Confirmatory testing required per ISO 11816-1:2013.
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