Question
The efficiency (adequacy) of pasteurization of milk is
tested bySolution
1. Science Behind the Test:Ā a. Alkaline phosphatase enzyme naturally present in raw milkĀ b. Destroyed at 72Ā°C for 15 sec (HTST standards)Ā c. Residual enzyme indicates inadequate pasteurization 2. Testing Protocol:Ā a. Add disodium phenyl phosphate + bufferĀ b. Incubate at 37Ā°C Ā± 1Ā°C for 1 hourĀ c. Measure liberated phenol (colorimetric) 3. Regulatory Standards:Ā a. FSSAI: ā¤ 350 mU/L for pasteurized milkĀ b. Codex Alimentarius: Complete inactivation required 4. Comparative Analysis:Ā 
5. Industry Practice:Ā a. Mandatory for all dairy processorsĀ b. Automated test systems (e.g., MilkoScan FT3) widely adopted Technical Note: False positives may occur if milk is contaminated with microbial phosphatases. Confirmatory testing required per ISO 11816-1:2013.
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