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Question

In two-dimensional electrophoresis, Proteins are separated

A First by their pl value and then by their mass Correct Answer Incorrect Answer
B First by their mass and then by their pl value Correct Answer Incorrect Answer
C Only on the basis of their charge Correct Answer Incorrect Answer
D Only on the basis of their mass Correct Answer Incorrect Answer

Solution

Two-dimensional gel electrophoresis (2-DE) is a key tool for comparative proteomics research. In 2-DE, mixtures of proteins are separated by charge (isoelectric point, pI) in the first dimension and further separated by mass in the second dimension on 2-D gels.

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