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      Question

      Which chemical is most commonly used for the 'Surface

      Sterilization' of explants?
      A Sugar solution Correct Answer Incorrect Answer
      B Mercuric Chloride Correct Answer Incorrect Answer
      C Distilled water Correct Answer Incorrect Answer
      D Auxin solution Correct Answer Incorrect Answer

      Solution

      Mercuric Chloride (or Sodium Hypochlorite) is used to kill surface-borne bacteria and fungi that would otherwise contaminate the nutrient-rich culture media. After treatment, the explant is rinsed thoroughly with sterile water.

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