Question
Arrange the steps of recombinant DNA technology in the
correct order: (A) Ligation (B) Transformation (C) Restriction digestion (D) Selection of transformed cellsSolution
The correct sequence is: Restriction digestion → Ligation → Transformation → Selection of transformed cells. The correct sequence of steps in recombinant DNA technology is: (i) Restriction digestion (C) – The desired gene and vector DNA are cut using restriction enzymes. (ii) Ligation (A) – The gene of interest is inserted into the vector using DNA ligase, forming recombinant DNA. (iii) Transformation (B) – The recombinant DNA is introduced into a host cell (e.g., bacteria). (iv) Selection of transformed cells (D) – Cells containing recombinant DNA are identified and selected using markers (e.g., antibiotic resistance genes).
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