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    Question

    Arrange the steps of recombinant DNA technology in the

    correct order: (A) Ligation (B) Transformation (C) Restriction digestion (D) Selection of transformed cells
    A A тЖТ B тЖТ C тЖТ D Correct Answer Incorrect Answer
    B C тЖТ A тЖТ B тЖТ D Correct Answer Incorrect Answer
    C B тЖТ A тЖТ C тЖТ D Correct Answer Incorrect Answer
    D C тЖТ B тЖТ A тЖТ D Correct Answer Incorrect Answer

    Solution

    The correct sequence is: Restriction digestion тЖТ Ligation тЖТ Transformation тЖТ Selection of transformed cells. The correct sequence of steps in recombinant DNA technology is: (i) Restriction digestion (C) тАУ The desired gene and vector DNA are cut using restriction enzymes. (ii) Ligation (A) тАУ The gene of interest is inserted into the vector using DNA ligase, forming recombinant DNA. (iii) Transformation (B) тАУ The recombinant DNA is introduced into a host cell (e.g., bacteria). (iv) Selection of transformed cells (D) тАУ Cells containing recombinant DNA are identified and selected using markers (e.g., antibiotic resistance genes).

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