Question
Arrange the steps of recombinant DNA technology in the
correct order: (A) Ligation (B) Transformation (C) Restriction digestion (D) Selection of transformed cellsSolution
The correct sequence is: Restriction digestion β Ligation β Transformation β Selection of transformed cells. The correct sequence of steps in recombinant DNA technology is: (i) Restriction digestion (C) β The desired gene and vector DNA are cut using restriction enzymes. (ii) Ligation (A) β The gene of interest is inserted into the vector using DNA ligase, forming recombinant DNA. (iii) Transformation (B) β The recombinant DNA is introduced into a host cell (e.g., bacteria). (iv) Selection of transformed cells (D) β Cells containing recombinant DNA are identified and selected using markers (e.g., antibiotic resistance genes).
- What approximate value will come in place of the question mark (?) in the following question? (Note: You are not expected to calculate the exact value.)
- What approximate value will come in place of the question mark (?) in the following question? (Note: You are not expected to calculate the exact value.)
5999.93 Γ· 60.005 Γ 70.002 = ? Γ 24.9
(1560.23 Γ· 25.98) + (768.32 Γ· 23.9) + 1814.11 = ?
40.22 of 249.98% + 459.99 Γ· 23.18 = ?
159.98% of 4820 + 90.33% of 2840 = ? + 114.99% of 1980
1587.9 + 9650.98 + 10612.8 =?3 - 2536.67
7.992 + (4.01 Γ 3.98) + ? = 224.03
(5.013 β 30.04) = ? + 11.98% of 4799.98
7.9% of 174.92 + 24.99 - 131.99Β Γ· 11.95 Γ 2.98 = ?